摘要 :
Fluorescence in situ hybridisation (FISH painting) has enabled the detection most of the stable radiation-induced chromosome aberrations (translocations, insertions). However, there is still no consensus about the use of FISH tech...
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Fluorescence in situ hybridisation (FISH painting) has enabled the detection most of the stable radiation-induced chromosome aberrations (translocations, insertions). However, there is still no consensus about the use of FISH techniques for biological dose reconstruction. At IPSN, two cocktails of three whole human chromosomes paints were used for both in vitro and in vivo studies (i.e. 2,4 and 12 for humans and 1, 4, 13 for rhesus monkeys). For human analysis, three in vitro dose-effect relationships were established using the same irradiation source (γ rays, ~60Co, 0.1 Gyl.min~-1, 0.5 Gy. Min~-1 and 1 Gy.min~-1). In vitro, the relative importance of complex exchanges was found to be negligible below 3 Gy.
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